WIP Regulates Persistence of Cell Migration and Ruffle Formation in Both Mesenchymal and Amoeboid Modes of Motility

PLoS One

Yolanda R. Carrasco¹  Julia Saez de Guinoa¹  Francisco Wandosell²  Alejandra Bernardini³  Chiara Ragazzini³  Inmaculada Banon-Rodriguez³  Ines Maria Anton³  Estefania Fernandez³  Gareth E. Jones⁴ 

[1] Department of Immunology and Oncology, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain;Department of Molecular Neurobiology, Centro de Biología Molecular “Severo Ochoa” (CBM-UAM), Madrid, Spain;Department of Molecular and Cell Biology, Centro Nacional de Biotecnología (CNB-CSIC), Madrid, Spain;The Randall Division of Cell and Molecular Biophysics, King's College London, London, United Kingdom

关键词: Fibroblasts;    B cells;    Chemotaxis;    Cell migration;    Actins;    Cell motility;    Wiskott-Aldrich syndrome;    Cell staining;   

DOI  :  10.1371/journal.pone.0070364

学科分类：医学（综合）

来源: Public Library of Science

【 摘 要 】

The spatial distribution of signals downstream from receptor tyrosine kinases (RTKs) or G-protein coupled receptors (GPCR) regulates fundamental cellular processes that control cell migration and growth. Both pathways rely significantly on actin cytoskeleton reorganization mediated by nucleation-promoting factors such as the WASP-(Wiskott-Aldrich Syndrome Protein) family. WIP (WASP Interacting Protein) is essential for the formation of a class of polarised actin microdomain, namely dorsal ruffles, downstream of the RTK for PDGF (platelet-derived growth factor) but the underlying mechanism is poorly understood. Using lentivirally-reconstituted WIP-deficient murine fibroblasts we define the requirement for WIP interaction with N-WASP (neural WASP) and Nck for efficient dorsal ruffle formation and of WIP-Nck binding for fibroblast chemotaxis towards PDGF-AA. The formation of both circular dorsal ruffles in PDGF-AA-stimulated primary fibroblasts and lamellipodia in CXCL13-treated B lymphocytes are also compromised by WIP-deficiency. We provide data to show that a WIP-Nck signalling complex interacts with RTK to promote polarised actin remodelling in fibroblasts and provide the first evidence for WIP involvement in the control of migratory persistence in both mesenchymal (fibroblast) and amoeboid (B lymphocytes) motility.

【 授权许可】

CC BY