Paracrine signalling between keratinocytes and SVF cells results in a new secreted cytokine profile during wound closure

Stem Cell Research & Therapy

Short Report

Afshin Raouf¹  Stefan Balko¹  Evan Kerr²  Sarvesh Logsetti³  Ed Buchel³ 

[1] Department of Immunology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;CancerCare Manitoba Research Institute, CancerCare Manitoba, Winnipeg, MB, Canada;Department of Immunology, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;Max Rady College of Medicine, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;Department of Surgery, Rady Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada;

关键词: Wound healing;    Scratch assay;    Cell migration;    Keratinocytes;    Fibroblasts;    Cytokines;   

DOI  :  10.1186/s13287-023-03488-0

 received in 2023-03-22, accepted in 2023-09-01,  发布年份 2023

来源: Springer

【 摘 要 】

Stromal vascular fraction (SVF) cells, and the adipose-derived mesenchymal stem cells they contain, have shown enhanced wound healing in vitro and in vivo, yet their clinical application has been limited. In this regard, understanding the mechanisms that govern SVF-enhanced wound healing would improve their application in the clinic. Here, we show that the SVF cells and keratinocytes engage in a paracrine crosstalk during wound closure, which results in a new cytokine profile that is distinct from the cytokines regularly secreted by either cell type on their own. We identify 11 cytokines, 5 of which are not regularly secreted by the SVF cells, whose expressions are significantly increased during wound closure by the keratinocytes. This new cytokine profile could be used to accelerate wound closure and initiate re-epithelialization without the need to obtain the SVF cells from the patient.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 5

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