会议论文详细信息
19th Argentinean Bioengineering Society Congress
Wound healing assay in a low-cost microfluidic platform
Conde, A.J.^1 ; Salvatierra, E.^2 ; Podhajcer, O.^2 ; Fraigi, L.^3 ; Madrid, R.E.^1
Laboratorio de Medios e Interfases, FACEyT, INSIBIO-CONICET, Av. Independencia 1800, S. M. de Tucumán 4000, Argentina^1
Fundación Instituto Leloir, Buenos Aires, Argentina^2
Instituto Nacional de Tecnología Industrial, San-Martín, Argentina^3
关键词: Cell migration;    Immune response;    Melanoma cells;    Microfluidic channel;    Microfluidic platforms;    Pathological process;    Vascularisation;    Wound healing assays;   
Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/477/1/012035/pdf
DOI  :  10.1088/1742-6596/477/1/012035
来源: IOP
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【 摘 要 】

Cell migration is a very important stage in different pathological processes such as wound healing, vascularisation, immune response, tissue regeneration and cancer. Traditional wound healing assays are done by scratching a monolayer on standard culture surfaces. However this method can lead to data misinterpretation and poor comparability in results from different experiments. The microfluidic wound healing assay is a mature alternative to the traditional method, but it is expensive and bulky due to the need of syringe pumps and can be difficult to implement by the end user. Here we show an alternative low-cost microfluidic platform based on the MainSTREAM platform. We also demonstrate the feasibility of a microperistaltic pump to carry out these kind of assays. A laminar flow of PBS-Trypsin-PBS confined in a microfluidic channel with a previously cultured monolayer of IIB-Mel LES melanoma cells was used to generate the wound. Cell migration rate could be quantified successfully.

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