Top-and-side dual-view microfluidic device with embedded prism

SENSORS AND ACTUATORS B-CHEMICAL

卷:248

Article

Kazama, Yuto¹  Carlen, Edwin T.^2,3  van den Berg, Albert³  Hibara, Akihide^4,5 

[1] Univ Tokyo, Inst Ind Sci, Tokyo, Japan

[2] Univ Tsukuba, Grad Sch Pure & Appl Sci, Tsukuba, Ibaraki, Japan

[3] Univ Twente, MESA, Enschede, Netherlands

[4] Tohoku Univ, Inst Multidisciplinary Res Adv Mat, Sendai, Miyagi, Japan

[5] Tokyo Inst Technol, Grad Sch Sci, Dept Chem, Tokyo, Japan

关键词: Dual view;    Microfluidic channel;    PDMS;    Dark-field view;    Fluorescence;   

DOI  :  10.1016/j.snb.2017.04.013

来源: Elsevier

【 摘 要 】

A polydimethylsiloxane microfluidic device enabling dual-view visualization is proposed and demonstrated. A prism with a 2 mm square base was embedded beside a 300 mu m-wide microchannel. In addition to ordinary visualization from the top of the device, the microchannel could be viewed from the side, and its optical path was reflected to the top by the prism. The top and side dual visualization in a single field of view was then realized with a single objective lens. The shifts in the focal point in the top and side directions were modeled, and a compensation method utilizing a flat sheet was used. After simultaneous bright-field and dark-field visualization was attained, dual-view fluorescence imaging of the fluorescent solution and cells was realized. (C) 2017 Elsevier B.V. All rights reserved.

【 授权许可】

Free