Whole genome sequence analysis of unidentified genetically modified papaya for development of a specific detection method

卷:205

Nakamura, Kosuke ; Kondo, Kazunari ; Akiyama, Hiroshi ; Ishigaki, Takumi ; Noguchi, Akio ; Katsumata, Hiroshi ; Takasaki, Kazuto ; Futo, Satoshi ; Sakata, Kozue ; Fukuda, Nozomi ; Mano, Junichi ; Kitta, Kazumi ; Tanaka, Hidenori ; Akashi, Ryo ; Nishimaki-Mogami, Tomoko

Natl Inst Hlth Sci

关键词: Genetically modified;    Transgenic sequence;    Next-generation sequencer;    Genome sequence;    Real-time PCR;    Carica papaya L.;   

DOI  :  10.1016/j.foodchem.2016.02.157

学科分类：食品科学和技术

【 摘 要 】

Identification of transgenic sequences in an unknown genetically modified (GM) papaya (Carica papaya L.) by whole genome sequence analysis was demonstrated. Whole genome sequence data were generated for a GM-positive fresh papaya fruit commodity detected in monitoring using real-time polymerase chain reaction (PCR). The sequences obtained were mapped against an open database for papaya genome sequence. Transgenic construct-and event-specific sequences were identified as a GM papaya developed to resist infection from a Papaya ringspot virus. Based on the transgenic sequences, a specific real-time PCR detection method for GM papaya applicable to various food commodities was developed. Whole genome sequence analysis enabled identifying unknown transgenic construct-and event-specific sequences in GM papaya and development of a reliable method for detecting them in papaya food commodities. (C) 2016 Elsevier Ltd. All rights reserved.