Determination of inorganic elements in blood of mice immunized with Bothrops Snake venom using XRF and NAA

37th Brazilian Meeting on Nuclear Physics

Da Silva, L. F. F. Lopes^1 ; Zamboni, C.B.^1 ; Bahovschi, V.^1 ; Metairon, S.^1 ; Suzuki, M.F.^1 ; Sant'Anna, O.A.^2 ; Rizzutto, M.A.^3

Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP), Av. Professor Lineu Prestes 2242, São Paulo, SP

05508-000, Brazil^1

Instituto Butantan, Av. Dr.Vital Brasil 1500, São Paulo, SP

05503-900, Brazil^2

Instituto de Física, Universidade de São Paulo, IFUSP, SP Rua: do Matão Travessa R 187, São Paulo, SP

05508-090, Brazil^3

关键词: Antibody response;    Control groups;    Energy-dispersive x-ray fluorescence techniques;    Genetically modified;    Human being;    Inorganic elements;    Snake venoms;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/630/1/012005/pdf
DOI  :  10.1088/1742-6596/630/1/012005

来源: IOP

【 摘 要 】

In this work, mice genetically modified [HIIIline] were immunized against different Bothrops snake venoms to produce anti-Bothrops serum (antivenom). The Neutron Activation Analysis (NAA) and Energy Dispersive X-Ray Fluorescence (EDXRF) techniques were used to evaluate Ca and Fe concentrations in blood of these immunized mice in order to establish a potential correlation between both phenotypes: antibody response and blood constituents after Bothrops venom administration. The results were compared with the control group (mice not immunized) and with human being estimative. These data are important for clinical screening of patients submitted to immunological therapy as well as the understanding of the envenoming mechanisms.