Chronic Rhinosinusitis: Potential Role of Microbial Dysbiosis and Recommendations for Sampling Sites

Frontiers in Cellular and Infection Microbiology

Naidoo, Yuresh¹  Forer, Martin²  Kong, Justin²  Leonard, Katherine³  Carney, Richard⁴  Woodcock, Stephen⁴  Burke, Catherine M.⁴  Oliver, Brian G. G.⁵  Stow, Nicholas W.⁵  Copeland, Elizabeth⁵  Seymour, Justin R.⁶ 

[1] Department of Otorhinolaryngology, Concord Hospital, University of Sydney, Australia;Department of Otorhinolaryngology, Royal North Shore Hospital, University of Sydney, Australia;Sydney Centre for Ear Nose and Throat, Frenchs Forest, Australia;The Climate Change Cluster, University of Technology Sydney, Australia;The School of Life Sciences, University of Technology Sydney, Australia;Woolcock Institute of Medical Research, The University of Sydney, Australia

关键词: Chronic rhinosinusitis;    microbiome;    sinus;    16S rRNA gene sequencing;    Middle meatus;   

DOI  :  10.3389/fcimb.2018.00057

学科分类：生物科学（综合）

来源: Frontiers

【 摘 要 】

Chronic rhinosinusitis (CRS) is an inflammatory condition that affects up to 12% of the human population in developed countries. Previous studies examining the potential role of the sinus bacterial microbiota within CRS infections have found inconsistent results, possibly because of inconsistencies in sampling strategies. The aim of this study was to determine whether the sinus microbiome is altered in CRS and additionally if the middle meatus is a suitable representative site for sampling the sinus microbiome. Swab samples were collected from 12 healthy controls and 21 CRS patients, including all eight sinuses for CRS patients and between one and five sinuses for control subjects. The left and right middle meatus and nostril swabs were also collected. Significant differences in the sinus microbiomes between CRS and control samples were revealed using high-throughput 16S rRNA gene sequencing. The genus Escherichia was over-represented in CRS sinuses, and associations between control patients and Corynebacterium and Dolosigranulum were also identified. Comparisons of the middle meatuses between groups did not reflect these differences, and the abundance of the Escherichia genus was significantly lower at this location. Additionally, intra-patient variation was lower between sinuses than between sinus and middle meatus, which together with the above results suggests that the middle meatus is not an effective representative sampling site

【 授权许可】

CC BY