Molecular identification of methane oxidizing bacteria from paddy soils and detection methane monooxygenase gene

International Symposium on Bioremediation, Biomaterial, Revegetation, and Conservation

生物科学;自然科学（总论）

Setiawan, R.^1 ; Sudiana, I.M.^1

Research Center for Biology, Indonesian Institute of Sciences, Cibinong

16911, Indonesia^1

关键词: 16S rRNA gene;    16S rRNA gene sequencing;    Methane emissions;    Methane monooxygenase;    Methane oxidizing bacterium;    Molecular identification;    Phylogenetic analysis;    Sequence similarity;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/308/1/012016/pdf
DOI  :  10.1088/1755-1315/308/1/012016

来源: IOP

【 摘 要 】

Methane uptake and production could take place in paddy soil. Methane oxidizing bacteria are the group of bacteria that play role in the methane uptake cycle and known as methanotroph. Methanotroph possess methane monooxygenase that could oxidize methane and short chain haloalkane. Methanotroph in paddy soils play role in controlling methane emission. Identification conducted in order to identify methanotroph from paddy soils. In this study, 5 methane oxidizing bacteria from paddy soils have been identified based on 16S rRNA gene sequencing and pmoA gene encoding particulate methane monooxygenase sequence. Phylogenetic analysis deduced from 16S rRNA gene and pmoA gene showed that 4 bacteria were identified as Methylophilus leisingeri with sequence similarity 99% and pmoA like type II methanotoph, while 1 bacterium was identified as Methylomonas koyamae with sequence similarity 99% and pmoA like type I methanoroph.