Identification and Antibacterial Activity of Bacteria Isolated from Marine Sponge Haliclona (Reniera) sp. against Multi-Drug Resistant Human Pathogen

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Asagabaldan, Meezan Ardhanu^1 ; Ayuningrum, D.^1 ; Kristiana, R.^1 ; Sabdono, A.^2 ; Radjasa, O.K.^2,3 ; Trianto, A.^2

Department of Coastal Resources Management, Diponegoro University, St. Imam Bardjo, SH, No. 15, Semarang

50241, Indonesia^1

Department of Marine Science, Faculty of Fisheries and Marine Science, Diponegoro University, St. Prof. H. Soedharto, SH, Tembalang, Semarang

50275, Indonesia^2

Ministry of Research, Technology and Higher Education, Indonesia^3

关键词: 16S rRNA gene sequencing;    Anti-bacterial activity;    antibacterial;    Enterobacter cloaceae;    Haliclona (Reniera) sp;    Multi-Drug Resistant;    Phylogenetic characterization;    Pseudomonas aeruginosa;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012019/pdf
DOI  :  10.1088/1755-1315/55/1/012019

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The marine sponge Haliclona (Reniera) sp. was a potential source of natural bioactive compounds. This sponge widely distributed along the coast of Panjang Island, Jepara, Indonesia. The aims of this research were to isolate the associated bacteria with Haliclona (Reniera) sp. and to screen the antibacterial activity against Multi-Drug Resistant (MDR) bacteria. Amount five bacteria were isolated using media selective for bacteria. The antibacterial activities of bacteria were performed by overlay methods. The bacteria strain PSP. 39-04 had the best activity against Pseudomonas aeruginosa, Staphylococcus aureus, Acinetobacter baumannii, and Enterobacter cloaceae. Based on colony morphology and phylogenetic characterization using 16S rRNA gene sequencing, PSP 39-04 was closely related with Chromohalobacter salixigens strain DSM3043.