Antioxidant and antibacterial activities of secondary metabolite endophytic bacteria from papaya leaf (Carica papaya L.)

13th Joint Conference on Chemistry

Sarjono, Purbowatiningrum Ria^1 ; Putri, Leni Diah^1 ; Budiarti, Chlara Eka^1 ; Mulyani, Nies Suci^1 ; Ngadiwiyana^1 ; Ismiyarto^1 ; Kusrini, Dewi^1 ; Adiwibawa Prasetya, Nor Basid^1

Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University Jl. Prof. Soedarto, SH Semarang

50275, Indonesia^1

关键词: Anti-bacterial activity;    Anti-oxidant activities;    antibacterial;    Bacterial isolation;    Endophytic bacteria;    Free radical scavenging activity;    Metabolite compounds;    Secondary metabolites;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/509/1/012112/pdf
DOI  :  10.1088/1757-899X/509/1/012112

来源: IOP

【 摘 要 】

Endophytic bacteria are capable of producing secondary metabolite compounds similar their host plants that can act as antioxidants and antibacterial. Previous research on endophytic bacterial isolation from papaya leaf (Carica Papaya L.) obtained 5 consortiums of endophytic bacteria symbiotic with papaya leaf. One of them is EC-3 isolate which are bacillus and gram positive. From this research, antioxidant activity test, antibacterial and extracellular content of EC-3 isolate metabolite compounds were conducted. The isolates of endophytic bacteria that symbiotes with the papaya leaves are able to produce alkaloid, flavonoids, saponins, tannins and triterpenoids compounds that act as the antioxidant and antibacterial. These isolates were selected from carica papaya leaves have antioxidant and antibacterial potential of secondary metabolite.EC-3 of secondary metabolite has the free radical scavenging activity of 68.5% and total phenolic as much as 69 mg greq gallic acid/gram sample. This value indicates that in 1 gram of metabolite extract, there is a phenolic compound equivalent to 69 mg of gallic acid. EC-3 secondary metabolites enhances the antibacterial potential to inhibited S. aureus, S. Typhi and E. coli.