Antioxidant and antibacterial activity of solid-liquid and enzyme-assisted extraction of phenolic compound from three species of tropical Sargassum

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Puspita, M.^1,2 ; Deniel, M.^1,3 ; Widowati, I.^2 ; Radjasa, O.K.^2 ; Douzenel, P.^1 ; Bedoux, G.^1 ; Bourgougnon, N.^1

University of South Brittany, EA 3884, LBCM, IUEM, Vannes

F-56000, France^1

University of Diponegoro, Faculty of Fisheries and Marine Science, Marine Science Department, Semarang

50275, Indonesia^2

University of West Brittany, Technopôle Brest-Iroise, Rue Dumont d'Urville, Plouzané

29280, France^3

关键词: Anti-bacterial activity;    Anti-oxidant activities;    Brown algae;    Conventional extraction;    Enzyme-assisted extractions;    Polyphenols;    Solid-liquid extraction;    Total phenolic content;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012057/pdf
DOI  :  10.1088/1755-1315/55/1/012057

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Sargassum has been well acknowledged for the potential natural product of its phlorotannins. Solid-liquid extraction (SLE) is the most common method used to extract them. However, this method has some drawbacks such as low yield and toxic. An alternative ecofriendly method has been proposed, i.e. enzyme-assisted extraction (EAE), proven to be more efficient. The aim is to compare the efficiency of SLE and EAE concerning their extraction yield, total phenolic content and antioxidant activity. S. aquifolium, S. ilicifolium and S. polycystum were extracted using water, methanol, methanol 50%, and ethanol 75% and enzymes (Viscozyme and Protamex). Total phenolic content (TPC) was analyzed by Folin-Ciocalteu and antioxidant activity via DPPH and FRAP analysis. This study implied that bioactivity of Sargassum extracted with enzymes is better compared to the one using organic solvents.