SIMULTANEOUS ESTIMATION OF ESOMEPRAZOLE AND LEVOSULPIRIDE IN BULK AND IN CAPSULE FORMULATION BY RP-HPLC

Journal of The Chilean Chemical Society

JAIN, RAKESHR¹  BARI, SANJAYB¹  PATIL, PRAVINO¹ 

[1] H.R. Patel Institute of Pharmaceutical Education and Research, Dhule, India

关键词: RP-;    HPLC;    Esomeprazole;    Levosulpiride;    Capsule Formulation;    Validation.;   

DOI  :  10.4067/S0717-97072013000300011

学科分类：化学（综合）

来源: Sociedad Chilena de Quimica

【 摘 要 】

The present work deals with developmentand validation for simultaneous determination of Esomeprazole and Levosulpiridedrugs in pharmaceutical formulations. A rapid, precise and specific reversephase high performance liquid chromatography (RP-HPLC) method was developedfor Esomeprazole and Levosulpiride. Chromatographic separations was achievedon a C-18 (5μm, 250x4.6 mm) HPLC column within a runtime of 10 min. Isocraticmobile phase contain methanol: buffer (pH 3) (65:35% v/v) and flow ratewas maintained at 1.0 mL/min. Eluate was monitored at 260 nm. Levosulpiridewas eluated at 2.7 min and Esomeprazole at 5.7 min. Linearity was studied inthe concentratiosn range of 5 to 30 μg mL-1 and 10 to 60 μg/ mL for esomeprazoleand levosulpiride respectively, with a correlation coefficient of 0.9995 and0.9993 respectively. The method was validated according to the ICH guidelineswith respect to specificity, linearity, accuracy, precision and robustness.

【 授权许可】

Unknown