Isolation, identification, and complete genome sequence of a bovine adenovirus type 3 from cattle in China

Virology Journal

Fei Xue¹  Chuang Lu¹  Qing-Feng Meng²  Hong-Fei Shi¹  Zhao-Li Li¹  Xiu-Mei Dong¹  Yu-Ran Gao¹  Hong Cai¹  Zuo Yu¹  Yuan-Mao Zhu¹ 

[1] Division of Livestock Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, No. 427 Maduan Street, Nan Gang District, Harbin 150001, Heilongjiang Province, PR China;Department of Ophthalmology, First Affiliated Hospital of Harbin Medical University, Heilongjiang, Harbin, China

关键词: DNA binding protein;    Complete genome;    Cattle;    Bovine adenovirus type 3;   

Others  :  1155365
DOI  :  10.1186/1743-422X-8-557

 received in 2011-07-21, accepted in 2011-12-22,  发布年份 2011

【 摘 要 】

Background

Bovine adenovirus type 3 (BAV-3) belongs to the Mastadenovirus genus of the family Adenoviridae and is involved in respiratory and enteric infections of calves. The isolation of BAV-3 has not been reported prior to this study in China. In 2009, there were many cases in cattle showing similar clinical signs to BAV-3 infection and a virus strain, showing cytopathic effect in Madin-Darby bovine kidney cells, was isolated from a bovine nasal swab collected from feedlot cattle in Heilongjiang Province, China. The isolate was confirmed as a bovine adenovirus type 3 by PCR and immunofluorescence assay, and named as HLJ0955. So far only the complete genome sequence of prototype of BAV-3 WBR-1 strain has been reported. In order to further characterize the Chinese isolate HLJ0955, the complete genome sequence of HLJ0955 was determined.

Results

The size of the genome of the Chinese isolate HLJ0955 is 34,132 nucleotides in length with a G+C content of 53.6%. The coding sequences for gene regions of HLJ0955 isolate were similar to the prototype of BAV-3 WBR-1 strain, with 80.0-98.6% nucleotide and 87.5-98.8% amino acid identities. The genome of HLJ0955 strain contains 16 regions and four deletions in inverted terminal repeats, E1B region and E4 region, respectively. The complete genome and DNA binding protein gene based phylogenetic analysis with other adenoviruses were performed and the results showed that HLJ0955 isolate belonged to BAV-3 and clustered within the Mastadenovirus genus of the family Adenoviridae.

Conclusions

This is the first study to report the isolation and molecular characterization of BAV-3 from cattle in China. The phylogenetic analysis performed in this study supported the use of the DNA binding protein gene of adenovirus as an appropriate subgenomic target for the classification of different genuses of the family Adenoviridae on the molecular basis. Meanwhile, a large-scale pathogen and serological epidemiological investigations for BVA-3 infection might be carried out in cattle in China. This report will be a good beginning for further studies on BAV-3 in China.

【 授权许可】

   
2011 Zhu et al; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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