Isolation and genotyping of viable Toxoplasma gondii from sheep and goats in Ethiopia destined for human consumption

Particle and Fibre Toxicology

Daniel Ajzenberg⁶  Marie-Laure Dardé⁶  Stephane De Craeye¹  Pierre Dorny²  Bruno Goddeeris⁴  Eric Cox⁸  Getachew Tilahun⁹  Tesfaye Sisay Tessema⁷  Mukarim Abdurahaman⁵  Endrias Zewdu Gebremedhin³ 

[1] Scientific Institute of Public Health, Communicable and Infectious Diseases, National Reference Center for Toxoplasmosis, Engelandstraat 642, Brussels B1180, Belgium;Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp B2000, Belgium;Faculty of Agriculture and Veterinary Sciences, Department of Veterinary Laboratory Technology, Ambo University, P. O. Box 19, Ambo, Ethiopia;Department of Biosystems, Faculty of Bioscience Engineering, Catholic University of Leuven, Kasteelpark Arenberg 30 bus 2456, Heverlee B-3001, Belgium;College of Agriculture and Veterinary Medicine, Jimma University, P. O. Box 307, Jimma, Ethiopia;Centre National de Référence (CNR) Toxoplasmose / Toxoplasma Biological Resource Center (BRC), Centre Hospitalier-Universitaire Dupuytren, Limoges 87042, France;Institute of Biotechnology, College of Natural and Computational Sciences, Unit of Health Biotechnology, Addis Ababa University, P. O. Box. 1176, Addis Ababa, Ethiopia;Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke B-9820, Belgium;Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P. O. Box. 1176, Addis Ababa, Ethiopia

关键词: Virulent;    Atypical;    Microsatellite;    Genotyping;    Goat;    Sheep;    Ethiopia;    DAT;    Bioassay;    Toxoplasma gondii;   

Others  :  1150399
DOI  :  10.1186/1756-3305-7-425

 received in 2014-08-03, accepted in 2014-08-28,  发布年份 2014

【 摘 要 】

Background

Toxoplasma gondii is an obligate intracellular protozoan parasite that infects humans and a broad spectrum of warm-blooded vertebrates. The present study was undertaken with the objectives of isolation and determining the genotypes of T. gondii strains from sheep and goats slaughtered in East and West Shewa Zones of Oromia Regional State, Central Ethiopia.

Methods

Hearts of 47 sheep and 44 goats that were seropositive in the Direct Agglutination Test (DAT) were bioassayed in mice. A multiplex PCR assay with 15 microsatellite markers was employed for genotyping of T. gondii isolates from sheep and goats.

Results

Viable T. gondii were isolated from 47 (51.65%) animals, 27 sheep and 20 goats. Most isolates caused sub-clinical infections in mice, however, 2 sheep and 1 goat isolates were mouse-virulent, killing mice between 19–27 days post-inoculation. The success of T. gondii isolation in mice increased significantly (P = 0.0001) with higher DAT antibody titers in sheep and goats. Genotyping revealed that 29 (87.88%) of the 33 isolates were Type II, 3 (9.09%) were Type III and 1 (3.03%) was atypical. Three strains (one type II, one type III, and the atypical genotype) were virulent for mice.

Conclusions

T. gondii tissue cysts in sheep and goats slaughtered for human consumption are widespread. This is the first report on isolation and genotyping of T. gondii from sheep and goats of Ethiopia.

【 授权许可】

   
2014 Gebremedhin et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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