Vector sequence contamination of the Plasmodium vivax sequence database in PlasmoDB and In silico correction of 26 parasite sequences

Particle and Fibre Toxicology

Qi Gao²  Hui Xia¹  Qiang Fang¹  Richard Culleton³  Cao Jun²  Xu Sui²  Zhi-Yong Tao¹ 

[1] Department of Parasitology, Bengbu Medical College, Bengbu, People’s Republic of China;Jiangsu Provincial Key Laboratory of Parasite Molecular Biology, Wuxi, China;Malaria Unit, Institute of Tropical Medicine, Nagasaki University, Sakamoto, Nagasaki, Japan

关键词: Genome;    Cloning vector;    Plasmodium vivax;    PlasmoDB;   

Others  :  1224203
DOI  :  10.1186/s13071-015-0927-x

 received in 2015-04-22, accepted in 2015-06-02,  发布年份 2015

【 摘 要 】

We found a 47 aa protein sequence that occurs 17 times in the Plasmodium vivax nucleotide database published on PlasmoDB. Coding sequence analysis showed multiple restriction enzyme sites within the 141 bp nucleotide sequence, and a His6 tag attached to the 3’ end, suggesting cloning vector origins. Sequences with vector contamination were submitted to NCBI, and BLASTN was used to cross-examine whole-genome shotgun contigs (WGS) from four recently deposited P. vivax whole genome sequencing projects. There are at least 26 genes listed in the PlasmoDB database that incorporate this cloning vector sequence into their predicted provisional protein products.

【 授权许可】

   
2015 Tao et al.

【 图 表 】

Fig. 1.

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