Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria

BMC Microbiology

Methodology Article

Krzysztof Glabski¹  Aneta A. Bartosik¹  Grazyna Jagura-Burdzy¹  Ewa Lewicka¹  Anna Kulinska²  Jolanta Godziszewska³  Aleksandra Markowska⁴ 

[1] Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland;Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland;Present address: Faculty of Chemistry, Institute of Biotechnology, Warsaw University of Technology, Warsaw, Poland;Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland;Present address: Faculty of Human Nutrition and Consumer Sciences, Laboratory of Food Chemistry, Warsaw University of Life Sciences, Warsaw, Poland;Department of Microbial Biochemistry, Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106, Warsaw, Poland;Present address: Pulawska 255A/4, 02-740, Warsaw, Poland;

关键词: Broad-host-range;    Cloning vector;    RK2;    lacZ;    xylE;    Stability functions;   

DOI  :  10.1186/s12866-016-0674-y

 received in 2015-11-04, accepted in 2016-03-17,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundLow-copy-number vectors of potential wide application in biotechnology need to encode stabilization modules ensuring their stable inheritance. The efficiency of stabilization may vary depending on the plasmid host so a thorough analysis of stabilization functions is required before use.ResultsTo facilitate such analysis highly unstable, mobilizable, broad-host-range (BHR) vectors based on RK2 replicon were constructed. The vectors are suitable for testing of various stabilization functions, including plasmid and chromosomal partitioning cassettes encoding ParB homologues capable of spreading on DNA. The xylE or lacZ reporter systems facilitate easy monitoring of plasmid segregation.ConclusionThe range of BHR vectors with different reporter cassettes and alternative mobilization systems expands their application in diverse bacterial species.

【 授权许可】

CC BY   
© Bartosik et al. 2016