Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo

Proteome Science

Shengwang Liu²  Xiangang Kong²  Demin Yu²  Junfeng Sun²  Xiaoli Liu²  Yuhao Shao²  Zongxi Han²  Zhongzan Cao¹ 

[1]College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, People's Republic of China

[2]Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, Harbin 150001, People's Republic of China

关键词: Kidney;    Trachea;    Chicken;    Proteomics;    Infectious bronchitis virus;   

Others  :  817317
DOI  :  10.1186/1477-5956-10-24

 received in 2011-12-09, accepted in 2012-03-31,  发布年份 2012

【 摘 要 】

Background

Infectious bronchitis virus (IBV) is first to be discovered coronavirus which is probably endemic in all regions with intensive impact on poultry production. In this study, we used two-dimensional gel electrophoresis (2-DE) and two-dimensional fluorescence difference gel electrophoresis (2-DIGE), coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS), to explore the global proteome profiles of trachea and kidney tissues from chicken at different stages infected in vivo with the highly virulent ck/CH/LDL/97I P₅ strain of infectious bronchitis virus (IBV) and the embryo-passaged, attenuated ck/CH/LDL/97I P₁₁₅ strain.

Results

Fifty-eight differentially expressed proteins were identified. Results demonstrated that some proteins which had functions in cytoskeleton organization, anti-oxidative stress, and stress response, showed different change patterns in abundance from chicken infected with the highly virulent ck/CH/LDL/97I P₅ strain and those given the embryo-passaged, attenuated P₁₁₅ stain. In addition, the dynamic transcriptional alterations of 12 selected proteins were analyzed by the real-time RT-PCR, and western blot analysis confirmed the change in abundance of heat shock proteins (HSP) beta-1, annexin A2, and annexin A5.

Conclusions

The proteomic alterations described here may suggest that these changes to protein expression correlate with IBV virus' virulence in chicken, hence provides valuable insights into the interactions of IBV with its host and may also assist with investigations of the pathogenesis of IBV and other coronavirus infections.

【 授权许可】

   
2012 Cao et al; licensee BioMed Central Ltd.

【 图 表 】

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