Glomerular proteomic profiling reveals early differences between preexisting and de novo type 2 diabetes in human renal allografts

BMC Nephrology

Research Article

Sigrid Nakken¹  Anne Kipp¹  Bjørn Egil Vikse²  Jessica Furriol³  Hans-Peter Marti³  Sabine Leh⁴  Janka Babickova⁵  Thea A. S. Halden⁶  Anders Åsberg⁷  Trond Jenssen⁸  Giulio Spagnoli⁹ 

[1] Department of Clinical Medicine, University of Bergen, Bergen, Norway;Department of Clinical Medicine, University of Bergen, Bergen, Norway;Department of Medicine, Haugesund Hospital, Haugesund, Norway;Department of Clinical Medicine, University of Bergen, Bergen, Norway;Department of Medicine, Haukeland University Hospital, Bergen, Norway;Department of Clinical Medicine, University of Bergen, Bergen, Norway;Department of Pathology, Haukeland University Hospital, Bergen, Norway;Department of Clinical Medicine, University of Bergen, Bergen, Norway;Institute of Molecular Biomedicine, Faculty of Medicine, Comenius University, Bratislava, Slovakia;Department of Transplantation Medicine, Oslo University Hospital, Rikshospitalet and University of Oslo, Oslo, Norway;Department of Transplantation Medicine, Oslo University Hospital, Rikshospitalet and University of Oslo, Oslo, Norway;Department of Pharmacy, University of Oslo, Oslo, Norway;Department of Transplantation Medicine, Oslo University Hospital, Rikshospitalet and University of Oslo, Oslo, Norway;Metabolic and Renal Research Group, Faculty of Health Sciences UiT, The Arctic University of Norway, Tromsø, Norway;Institute of Translational Pharmacology, National Research Council, Rome, Italy;

关键词: PTDM;    T2DM;    Proteomics;    Tissue transplantation;    Kidney;   

DOI  :  10.1186/s12882-023-03294-z

 received in 2022-06-28, accepted in 2023-08-08,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundDiabetes mellitus (DM), either preexisting or developing after transplantation, remains a crucial clinical problem in kidney transplantation. To obtain insights into the molecular mechanisms underlying PTDM development and early glomerular damage before the development of histologically visible diabetic kidney disease, we comparatively analysed the proteome of histologically normal glomeruli from patients with PTDM and normoglycaemic (NG) transplant recipients. Moreover, to assess specificities inherent in PTDM, we also comparatively evaluated glomerular proteomes from transplant recipients with preexisting type 2 DM (T2DM).MethodsProtocol biopsies were obtained from adult NG, PTDM and T2DM patients one year after kidney transplantation. Biopsies were formalin-fixed and embedded in paraffin, and glomerular cross-sections were microdissected. A total of 4 NG, 7 PTDM and 6 T2DM kidney biopsies were used for the analysis. The proteome was determined by liquid chromatography-tandem mass spectrometry. Relative differences in protein abundance and significantly dysregulated pathways were analysed.ResultsProteins involved in cell adhesion, immune response, leukocyte transendothelial filtration, and cell localization and organization were less abundant in glomeruli from PTDM patients than in those from NG patients, and proteins associated with supramolecular fibre organization and protein-containing complex binding were more abundant in PTDM patients. Overall, proteins related to adherens and tight junctions and those related to the immune system, including leukocyte transendothelial migration, were more abundant in NG patients than in transplanted patients with DM, irrespective of the timing of its development. However, proteins included in cell‒cell junctions and adhesion, insulin resistance, and vesicle-mediated transport were all less abundant in PTDM patients than in T2DM patients.ConclusionsThe glomerular proteome profile differentiates PTDM from NG and T2DM, suggesting specific pathogenetic mechanisms. Further studies are warranted to validate these results, potentially leading to an improved understanding of PTDM kidney transplant pathophysiology and to the identification of novel biomarkers.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

Fig. 2

Fig. 7

Fig. 5

Fig. 3

Fig. 1