Selection of recombinant MVA by rescue of the essential D4R gene

Virology Journal

Georg W Holzer²  Falko G Falkner²  Thomas R Kreil¹  Birgit Schäfer²  Patricia S Ricci² 

[1] Baxter BioScience, 1221 Vienna, Austria;Baxter BioScience, Biomedical Research Center, Uferstrasse 15, 2304 Orth an der Donau, Austria

关键词: development;    Defective viruses/growth &;    Defective viruses/genetics;    Transformed;    Cell Line;    Vaccinia virus;    Uracil-DNA glycosylase;    Recombinant vaccines;   

Others  :  1155545
DOI  :  10.1186/1743-422X-8-529

 received in 2011-09-14, accepted in 2011-12-12,  发布年份 2011

【 摘 要 】

Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant.

【 授权许可】

   
2011 Ricci et al; licensee BioMed Central Ltd.

【 图 表 】

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