Modulation of cytosolic protein kinase C activity by ferricyanide: priming event seems transmembrane redox signalling

FEBS Letters

Anglard, P.¹  Malviya, A.N.¹ 

[1] Centre de Neurochimie du CNRS, Université Louis Pasteur, 5 rue Blaise Pascal, 67084 Strasbourg Cedex, France

关键词: Protein kinase C Tumor promotion Transformed cell Transmembrane signaling Ferricyanide Na+/H+ antiporter;   

DOI  :  10.1016/0014-5793(86)81149-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transformed 3T3/10T1/2 cultured cells incubated with ferricyanide caused a decrease of 2 mM EDTA extractable cytosolic protein kinase C activity in 2 min, whereas 5 or 20 min ferricyanide treatment reverted the enzyme activity to that observed without ferricyanide. The ferricyanide effect in 2 min was abolished by amiloride and sustained by ouabain. Thus, deactivation-activation of cytosolic protein kinase C is attributed to an unknown signal generation during H⁺ accumulation coupled with the Na⁺/H⁺ exchange phase. In this mechanism the priming event concerns the transmembrane redox process shedding H⁺ into the cell interior while impermeant ferricyanide acts as a unique electron acceptor.

【 授权许可】

Unknown