The Proteome Profiles of the Cerebellum of Juvenile, Adult and Aged Rats—An Ontogenetic Study

International Journal of Molecular Sciences

Michael Wille²  Antje Schümann²  Andreas Wree²  Michael Kreutzer³  Michael O. Glocker³  Grit Mutzbauer¹  Oliver Schmitt² 

[1] Department of Pathology, Josef-Schneider-Str. 2, 97080 Würzburg, Germany; E-Mail:;Department of Anatomy, Gertrudenstr. 9, 18055 Rostock, Germany; E-Mails:;Proteome Center Rostock, Schillingallee 69, 18055 Rostock, Germany; E-Mails:

关键词: proteomics;    rat;    brain;    cerebellum;    development;   

DOI  :  10.3390/ijms160921454

来源: mdpi

【 摘 要 】

In this study, we searched for proteins that change their expression in the cerebellum (Ce) of rats during ontogenesis. This study focuses on the question of whether specific proteins exist which are differentially expressed with regard to postnatal stages of development. A better characterization of the microenvironment and its development may result from these study findings. A differential two-dimensional polyacrylamide gel electrophoresis (2DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis of the samples revealed that the number of proteins of the functional classes differed depending on the developmental stages. Especially members of the functional classes of biosynthesis, regulatory proteins, chaperones and structural proteins show the highest differential expression within the analyzed stages of development. Therefore, members of these functional protein groups seem to be involved in the development and differentiation of the Ce within the analyzed development stages. In this study, changes in the expression of proteins in the Ce at different postnatal developmental stages (postnatal days (P) 7, 90, and 637) could be observed. At the same time, an identification of proteins which are involved in cell migration and differentiation was possible. Especially proteins involved in processes of the biosynthesis and regulation, the dynamic organization of the cytoskeleton as well as chaperones showed a high amount of differentially expressed proteins between the analyzed dates.

【 授权许可】

CC BY   
© 2015 by the authors; licensee MDPI, Basel, Switzerland.