Effect of n-3 and n-6 unsaturated fatty acids on prostate cancer (PC-3) and prostate epithelial (RWPE-1) cells in vitro

Lipids in Health and Disease

Undurti N Das⁴  Shengrong Shen¹  Feng Zhou¹  Junhui Shen²  Yuzhen Shen¹  Hongzhou Meng³ 

[1] Department of Food Science and Nutrition, School of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China;School of Medicine, Tongji University, Shanghai 200092, China;Department of Urology, The First Affiliated Hospital, Zhejiang University, Hangzhou 310003, China;Department of Medicine, GVP Hospital and Bio-Science Research Centre, Campus of GVP College of Engineering, Visakhapatnam 530 048, India

关键词: Lipoxin A4;    Lipid peroxidation;    Free radicals;    Cytokines;    Prostate cancer;    n-3 Polyunsaturated fatty acids;    n-6 Polyunsaturated fatty acids;   

Others  :  829362
DOI  :  10.1186/1476-511X-12-160

 received in 2013-07-17, accepted in 2013-10-25,  发布年份 2013

【 摘 要 】

Prostate cancer (PCa) is one of the leading causes of death in the elderly men. Polyunsaturated fatty acids (PUFAs) regulate proliferation of cancer cells. In the present study, we evaluated the effect of various PUFAs on the proliferation and survival of human prostate cancer (PC-3) and human prostate epithelial (RWPE-1) cells in vitro.

LA, GLA, AA, ALA, EPA and DHA (linoleic acid, gamma-linolenic acid, arachidonic acid, alpha-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid respectively) when tested at 50, 100, 150, and 200 μM inhibited proliferation of RWPE-1 and PC-3 cells, except that lower concentrations of LA (25 μM) and GLA (5, 10 μM) promoted proliferation. Though all fatty acids tested produced changes in the production of interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), lipoxin A₄ and free radical generation by RWPE-1 and PC-3 cells, there were significant differences in their ability to do so. As expected, supplementation of various n-3 and n-6 fatty acids to RWPE-1 and PC-3 cells enhanced the content of the added fatty acids and their long-chain metabolites in these cells. In contrast to previous results, we did not find any direct correlation between inhibition of cell proliferation induced by various fatty acids and free radical generation. These results suggest that polyunsaturated fatty acids suppress proliferation of normal and tumor cells by a variety of mechanisms that may partly depend on the type(s) of cell(s) being tested and the way these fatty acids are handled by the cells. Hence, it is suggested that more deeper and comprehensive studies are needed to understand the actions of fatty acids on the growth of normal and tumor cells.

【 授权许可】

   
2013 Meng et al.; licensee BioMed Central Ltd.

【 图 表 】

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