Glucose and fatty acid metabolism in infarcted heart from streptozotocin-induced diabetic rats after 2 weeks of tissue remodeling

Cardiovascular Diabetology

Maria Claudia Irigoyen⁵  Rui Curi²  Fernanda Consolim-Colombo⁵  Kleiton Augusto Santos Silva³  Leandro Ezequiel de Souza⁴  Diego Figueroa⁴  Andressa Bolsoni-Lopes²  Mariana Carbonaro⁴  Alcione Lescano de Souza Junior¹  Christiane Malfitano² 

[1] Nursing Department, State University of Mato Grosso, Alta Floresta, Brazil;Department of Physiology and Biophysics, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil;Department of Nephrology, School of Medicine of the Federal, University of Sao Paulo, Sao Paulo, Brazil;Hypertension Unit, Heart Institute (InCor), Medical School of University of São Paulo, Av. Eneas de Carvalho Aguiar, 44, Sao Paulo, 05403-000, SP, Brazil;Laboratory of Translational Physiology, Universidade Nove de Julho, (UNINOVE), Sao Paulo, Brazil

关键词: Remodeling;    Myocardial infarction;    Diabetic hyperglycemia;    Fatty acid metabolism;    Glucose metabolism;   

Others  :  1231858
DOI  :  10.1186/s12933-015-0308-y

 received in 2015-08-05, accepted in 2015-10-23,  发布年份 2015

【 摘 要 】

Background

The effects of streptozotocin (STZ)-induced diabetes on heart metabolism and function after myocardial infarction (MI) remodelling were investigated in rats.

Methods

Fifteen days after STZ (50 mg/kg b.w. i.v.) injection, MI was induced by surgical occlusion of the left coronary artery. Two weeks after MI induction, contents of glycogen, ATP, free fatty acids and triacylglycerols (TG) and enzyme activities of glycolysis and Krebs cycle (hexokinase, glucose-6-phosphate dehydrogenase, phosphofructokinase, citrate synthase) and expression of carnitine palmitoyl-CoA transferase I (a key enzyme of mitochondrial fatty acid oxidation) were measured in the left ventricle (LV). Plasma glucose, free fatty acids and triacylglycerol levels were determined. Ejection fraction (EF) and shortening fraction (SF) were also measured by echocardiography.

Results

Glycogen and TG contents were increased (p < 0.05) whereas ATP content was decreased in the LV of the non-infarcted diabetic group when compared to the control group (p < 0.05). When compared to infarcted control rats (MI), the diabetic infarcted rats (DI) showed (p < 0.05): increased plasma glucose and TG levels, elevated free fatty acid levels and increased activity of, citrate synthase and decreased ATP levels in the LV. Infarct size was smaller in the DI group when compared to MI rats (p < 0.05), and this was associated with higher EF and SF (p < 0.05).

Conclusions

Systolic function was preserved or recovered more efficiently in the heart from diabetic rats two weeks after MI, possibly due to the high provision of glucose and free fatty acids from both plasma and heart glycogen and triacylglycerol stores.

【 授权许可】

   
2015 Malfitano et al.

【 图 表 】

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