Source tracking of human leptospirosis: serotyping and genotyping of Leptospira isolated from rodents in the epidemic area of Guizhou province, China

BMC Microbiology

Jie Yan³  Xiugao Jiang²  Guangpeng Tang¹  Jingzhu Zhou¹  Guanghai Yao¹  Ying Liu¹  Yixin Nie²  Xiuwen Li²  Kecheng Tian¹  Xiaoyu Wei¹  Cuicai Zhang²  Dingming Wang¹  Shijun Li¹ 

[1]Institute of Communicable Disease Control and Prevention, Guizhou Provincial Centre for Disease Control and Prevention, 73 Bageyan Road, Guiyang 550004, Guizhou, People's Republic of China

[2]National Institute for Communicable Disease Control and Prevention, Chinese Centre for Disease Control and Prevention, 155 Changbai Road, Changping District, 102206 Beijing, People's Republic of China

[3]Laboratory of Microbiology and Parasitology, College of Medicine, Zhejiang University, 388 Yuhangtang Road, Hangzhou 310058, Zhejiang, People's Republic of China

关键词: Source of infection;    Genotyping;    Serotyping;    Leptospira;    Rodents;   

Others  :  1143957
DOI  :  10.1186/1471-2180-13-75

 received in 2012-12-19, accepted in 2013-03-27,  发布年份 2013

【 摘 要 】

Background

Sustained human leptospirosis as well as death cases has been reported in Qiandongnan Prefecture, Southeast of Guizhou, China, recently, but these human patients were only clinically diagnosed, and leptospires have never been isolated from patients in these epidemic regions, In order to track the source of infection and understand the etiologic characteristic of leptospirosis, we performed rodent carrier surveillance for leptospirosis in the epidemic area in 2011. The population distribution of rodents in the epidemic regions was revealed.

Results

Four strains of leptospire were isolated from Apodemus agrarius. Microscopic agglutination test (MAT) confirmed the four isolates belonged to leptospiral serogroup Icterohaemorrhagiae. Multilocus sequence typing (MLST) indicated that all the four strains were defined as sequence type 1(ST1), which is identical to the three strains isolated from Rattus tanezumi in Rongjiang County in 2007. Clustering analysis of the MLST data indicated that the local isolates exactly matched with reference strain of leptospiral serovar Lai strain 56601, which is consistent with anti-Leptospira antibody detection of patients using MAT.

Conclusions

Apodemus agrarius may be the potentially important carrier of leptospirosis and the potential source of leptospiral infection in human, and serovar Lai maybe the epidemic serovar of Leptospira in the localities.

【 授权许可】

   
2013 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

Figure 2.

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