The Ecdysone receptor constrains wingless expression to pattern cell cycle across the Drosophila wing margin in a cyclin B-dependent manner

BMC Developmental Biology

Leonie M Quinn¹  Amanda Lee¹  Nicola Cranna¹  Olga Zaytseva¹  Jane I Lin¹  Naomi C Mitchell¹ 

[1] Department of Anatomy and Cell Biology, University of Melbourne, Parkville 3010, Melbourne, Australia

关键词: Wingless;    Cell cycle;    Drosophila;    Cyclin B;    Ecdysone;   

Others  :  1085624
DOI  :  10.1186/1471-213X-13-28

 received in 2013-03-25, accepted in 2013-07-10,  发布年份 2013

【 摘 要 】

Background

Ecdysone triggers transcriptional changes via the ecdysone receptor (EcR) to coordinate developmental programs of apoptosis, cell cycle and differentiation. Data suggests EcR affects cell cycle gene expression indirectly and here we identify Wingless as an intermediary factor linking EcR to cell cycle.

Results

We demonstrate EcR patterns cell cycle across the presumptive Drosophila wing margin by constraining wg transcription to modulate CycB expression, but not the previously identified Wg-targets dMyc or Stg. Furthermore co-knockdown of Wg restores CycB patterning in EcR knockdown clones. Wg is not a direct target of EcR, rather we demonstrate that repression of Wg by EcR is likely mediated by direct interaction between the EcR-responsive zinc finger transcription factor Crol and the wg promoter.

Conclusions

Thus we elucidate a critical mechanism potentially connecting ecdysone with patterning signals to ensure correct timing of cell cycle exit and differentiation during margin wing development.

【 授权许可】

   
2013 Mitchell et al.; licensee BioMed Central Ltd.

【 图 表 】

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