Combinatorial Signaling by an Unconventional Wg Pathway and the Dpp Pathway Requires Nejire (CBP/p300) to Regulate dpp Expression in Posterior Tracheal Branches

Developmental Biology

O.H. Sultani¹  N.T. Takaesu¹  A.N. Johnson¹  S.J. Newfeld¹ 

[1] Department of Biology, Arizona State University, Tempe, Arizona, 85287-1501

关键词: Drosophila;    Wingless;    Decapentaplegic;    Nejire (CBP;    p300);    combinatorial signaling;    dorsal ectoderm;    tracheal system;    posterior spiracle;   

DOI  :  10.1006/dbio.2002.0693

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Thedecapentaplegic(dpp)geneinfluencesmanydevelopmentaleventsinDrosophilamelanogaster.Wehavebeenanalyzingdppexpressionintwogroupsofdorsalectodermcellsattheposteriorendoftheembryo,inabdominalsegment8andthetelson.Thesedpp-expressingcellsbecometrachealcellsintheposterior-mostbranchesofthetrachealsystem(DorsalBranch10,SpiracularBranch10,andthePosteriorSpiracle).Thesebranchesarenotidentifiedbyreagentstypicallyusedinanalysesoftrachealdevelopment,suggestingthatdppexpressionconfersadistinctidentityuponposteriortrachealcells.Wehavedeterminedthatdppposteriorectodermexpressionbeginsduringgermbandextensionandcontinuesthroughoutdevelopment.Wehaveisolatedthesequencesresponsiblefortheseaspectsofdppexpressioninareportergene.WehavedeterminedthatanunconventionalformofWingless(Wg)signaling,Dppsignaling,andthetranscriptionalcoactivatorNejire(CBP/p300)arerequiredfortheinitiationandmaintenanceofdppexpressionintheposterior-mostbranchesofthetrachealsystem.OurdatasuggestamodelfortheintegrationofWgandDppsignalsthatmaybeapplicabletobranchingmorphogenesisinotherdevelopmentalsystems.

【 授权许可】

Unknown