Drosophila APC2 and APC1 Have Overlapping Roles in the Larval Brain Despite Their Distinct Intracellular Localizations

Developmental Biology

Mark Peifer¹  Kathryn Akong¹  Brooke M. McCartney¹ 

[1] Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, 27599-3280

关键词: APC;    β-catenin;    Armadillo;    brain;    Wingless;    Drosophila;    tumor suppressor;    neuroblast;    cadherin;    asymmetric division;   

DOI  :  10.1006/dbio.2002.0777

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

ThetumorsuppressorAPCanditshomologs,firstidentifiedforaroleincoloncancer,negativelyregulateWntsignalinginbothoncogenesisandnormaldevelopment,andplayWnt-independentrolesincytoskeletalregulation.BothDrosophilaandmammalshavetwoAPCfamilymembers.WefurtherexploredthefunctionsoftheDrosophilaAPCsusingthelarvalbrainasamodel.Wefoundthatbothproteinsareexpressedinthebrain.APC2hasahighlydynamic,asymmetriclocalizationthroughthelarvalneuroblastcellcyclerelativetoknownmediatorsofembryonicneuroblastasymmetricdivisions.Adherensjunctionproteinsalsoareasymmetricallylocalizedinneuroblasts.InadditiontheyaccumulatewithAPC2andAPC1innervesformedbyaxonsoftheprogenyofeachneuroblast-ganglionmothercellcluster.APC2andAPC1localizetoverydifferentplaceswhenexpressedinthelarvalbrain:APC2localizestothecellcortexandAPC1tocentrosomesandmicrotubules.Despitethis,theyplayredundantrolesinthebrain;whileeachsinglemutantisnormal,thezygoticdoublemutanthasseverelyreducednumbersoflarvalneuroblasts.OurexperimentssuggestthatthisdoesnotresultfrommisregulationofWgsignaling,andthusmayinvolvethecytoskeletaloradhesiverolesofAPCproteins.

【 授权许可】

Unknown