Drosophila APC2 and APC1 Play Overlapping Roles in Wingless Signaling in the Embryo and Imaginal Discs

Developmental Biology

Mark Peifer¹  Kathryn Akong¹  Brooke M. McCartney¹  Melissa A. Hayden¹  Elizabeth E. Grevengoed¹  Meredith H. Price¹  Jan C. DeNofrio¹ 

[1] Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina, 27599-3280

关键词: APC;    β-catenin;    Armadillo;    Wnt;    Wingless;    Drosophila;    tumor suppressor;   

DOI  :  10.1006/dbio.2002.0776

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Theregulationofsignaltransductionplaysakeyroleincellfatechoices,anditsdisregulationcontributestooncogenesis.ThisdualityisexemplifiedbythetumorsuppressorAPC.Originallyidentifiedforitsroleincolontumors,APCfamilymembersweresubsequentlyshowntonegativelyregulateWntsignalinginbothdevelopmentanddisease.TheanalysisofthenormalrolesofAPCproteinsiscomplicatedbythepresenceoftwoAPCfamilymembersinfliesandmice.Previousworkdemonstratedthat,insometissues,singlemutationsineachgenehavenoeffect,raisingthequestionofwhetherthereisfunctionaloverlapbetweenthetwoAPCsorwhetherAPC-independentmechanismsofWntregulationexist.WeaddressedthisbyeliminatingthefunctionofbothDrosophilaAPCgenessimultaneously.WefindthatAPC1andAPC2playoverlappingrolesinregulatingWinglesssignalingintheembryonicepidermisandtheimaginaldiscs.Surprisingly,APC1functioninembryosoccursatlevelsofexpressionnearlytoolowtodetect.Further,theoverlappingfunctionsexistdespitestrikingdifferencesintheintracellularlocalizationofthetwoAPCfamilymembers.

【 授权许可】

Unknown