A Transgenic Lef1/β-Catenin-Dependent Reporter Is Expressed in Spatially Restricted Domains throughout Zebrafish Development

Developmental Biology

Richard I. Dorsky¹  Laird C. Sheldahl¹  Randall T. Moon¹ 

[1] Howard Hughes Medical Institute, Department of Pharmacology and Center for Developmental Biology, University of Washington, Seattle, Washington, 98195

关键词: Wnt;    β-catenin;    Lef1;    Tcf3;    GFP;    reporter;    transgenic;    zebrafish;   

DOI  :  10.1006/dbio.2001.0515

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

TheWnt/β-cateninsignalingpathwayplaysmultiplerolesduringembryonicdevelopment,onlyafewofwhichhavebeenextensivelycharacterized.AlthoughdomainsofWntexpressionhavebeenidentifiedthroughoutembryogenesis,anatomicalandmolecularcharacterizationofrespondingcellshasbeenmostlyunexplored.Wehavegeneratedatransgeniczebrafishlinethatexpressesadestabilizedgreenfluorescentprotein(GFP)variantunderthecontrolofaβ-cateninresponsivepromoter.Earlyzygoticexpressionofthistransgene(TOPdGFP)mirrorsknowndomainsofWntsignalingintheembryo.LossofLef1activityresultsindecreasedreporterexpressionandposteriordefects,whilelossofTcf3(Headless,Hdl)activitydoesnotalterreporterexpression,eventhoughitresultsinlossofforebrainstructures.Inaddition,ectopicWnt1expressioncanactivatethereporter.Inolderembryos,weidentifyanumberoftransgene-expressingcellpopulationsasnovelsitesofβ-cateninsignaling.WeconcludethatourTOP-dGFPreporterlinefaithfullyillustratesdomainsofβ-cateninactivityandenablestheidentificationofresponsivecellpopulations.

【 授权许可】

Unknown