Biochemical characterization of the Drosophila Axin protein

FEBS Letters

Ishimoto, Akinori¹  Yanagawa, Shin-ichi¹  Lee, Jong-Seo¹  Matsuda, Yukihiro¹ 

[1] Department of Viral Oncology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto 606-8507, Japan

关键词: Axin;    Wingless;    Armadillo;    Zeste-white 3;    Dishevelled;    Drosophila;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/S0014-5793(00)01601-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the Wnt/Wingless pathway, accumulation of β-catenin/Armadillo protein is a key regulatory step. Vertebrate Axin is a negative regulator of Wnt signaling, promoting glycogen synthase kinase-3β-mediated phosphorylation of β-catenin and thereby destabilizing β-catenin. Using Drosophila cell culture systems, we demonstrated that a Drosophila homolog of Axin (Daxin) inhibits Wingless-induced Armadillo accumulation and Drosophila T-cell factor-dependent transcription induced by Wingless, Dishevelled, and Armadillo. The carboxy-terminal portion of Daxin is not essential for the inhibitory activity, but a mutant only consisting of this portion behaves as a dominant-negative protein. Moreover, interactions between Daxin and Zeste-white 3, Armadillo, Dishevelled, protein phosphatase 2A and Daxin itself were shown, providing direct evidence that Daxin is a scaffold protein in the Wingless pathway.

【 授权许可】

Unknown