Presence of intratumoral platelets is associated with tumor vessel structure and metastasis

BMC Cancer

Jianbo Wu³  Bing Ran²  Xiao Zhang¹  Zhuo Zhang¹  Xu Zhang¹  Bing He¹  Jinbo Liu¹  Guang Yu²  Jiyi Xia¹  Xin Deng¹  Mao Luo¹  Ni Chen¹  Meiping Ren¹  Rong Li¹ 

[1]Drug Discovery Research Center, Luzhou Medical College, Luzhou, Sichuan, People's Republic of China

[2]Department of Physiology, Luzhou Medical College, Luzhou, Sichuan, People's Republic of China

[3]Dalton Cardiovascular Research Center, University of Missouri, Research Park Dr., Columbia 652121, MO, USA

关键词: Angiogenesis;    Hypoxia;    Metastasis;    Tumorigenesis;    Platelets;   

Others  :  858989
DOI  :  10.1186/1471-2407-14-167

 received in 2014-01-27, accepted in 2014-02-28,  发布年份 2014

【 摘 要 】

Background

Platelets play a fundamental role in maintaining hemostasis and have been shown to participate in hematogenous dissemination of tumor cells. Abundant platelets were detected in the tumor microenvironment outside of the blood vessel, thus, platelet -tumor cell interaction outside of the bloodstream may play a role in regulating primary tumor growth and metastasis initiation. However, it is unclear that platelet depletion affects tumor vessel structure and dynamics.

Methods

Using thrombocytopenia induction in two different tumor-bearing mouse models, tumor tissues were performed by Westernblotting and immunohistochemical staining. Vascular permeability was evaluated by determination of intratumoral Evans blue and Miles vascular permeability assay. Furthermore, microdialysis was used to examining the intratumoral extracellular angiogenic growth factors (VEGF, TGF-β) by ELISA.

Results

Platelet depletion showed no change in tumor growth and reduced lung metastasis. Platelet depletion led to reduced tumor hypoxia and Met receptor activation and was associated with a decreased release of MMP-2, 9, PAI-1, VEGF, and TGF-β. Tumor vessels in platelet-depleted mice showed impaired vessel density and maturation.

Conclusions

Our findings demonstrate that platelets within the primary tumor microenvironment play a critical role in the induction of vascular permeability and initiation of tumor metastasis.

【 授权许可】

   
2014 Li et al.; licensee BioMed Central Ltd.

【 图 表 】

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