Induction of Angiogenesis Process in Mandible Using Anadara granosa Shell Graft (Experimental Laboratory Study on Rattus norvegicus)

12th Congress of Indonesian Soc. for Biochemistry and Molecular Biology in Conjunction With The 2nd Int. Conf. "Collaboration Seminar of Chemistry and Industry

生物科学;化学

Widyastuti, W.^1 ; Rubianto, M.^2 ; Soetjipto^3

Departement of Periodontia, Faculty of Dentistry, University of Hang Tuah, Indonesia^1

Departement of Periodontia, Faculty of Dentistry, Airlangga University, Indonesia^2

Departement of Biochemistry, Faculty of Medicine, Airlangga University, Indonesia^3

关键词: Angiogenesis;    Bone graft;    FGF-2;    healing;    VEGF;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/217/1/012033/pdf
DOI  :  10.1088/1755-1315/217/1/012033

学科分类：生物科学（综合）

来源: IOP

【 摘 要 】

Alveolar bone is a highly vascularized organ and angiogenesis plays an important role in osteogenesis. Vascular endothelial growth factor (VEGF) is an essential mediator during the process angiogenesis and osteogenic potential, fibroblast growth factor (FGF-2) either synergistically with VEGF, act on early stages of induction by stimulating fibroblast and osteoblast proliferations. A. granosa shell graft (AG) has osteoconductivity so it can be a scaffold to attract pluripotent cells that support bone healing process. Purpose: To improve bone blood flow and bone remodeling in mandible using A. granosa shell graft for accelerate osteogenesis. Methods: Total of 18 male rats Rattus norvegicus aged 6 months with body weight 250-300 grams were randomly devided into 3 groups with 6 rats each group. All samples got it's mandibular right incisors extracted, curettage and irrigation. The group consist of : Treatment group with AG, treatment group with xenograft, control group untreated graft. After one week rats were sacrificed for FGF-2 and VEGF analized with Oneway Anova. Results: AG shell graft containing 44% calcium carbonate, 33% calcium hydroxide and 23% calcium phosphate could increase the expression of FGF-2 and VEGF (p