Impact of the exopolysaccharide layer on biofilms, adhesion and resistance to stress in Lactobacillus johnsonii FI9785

BMC Microbiology

Arjan Narbad¹  Melinda J Mayer¹  Enes Dertli² 

[1] Gut Health and Food Safety Institute Strategic Programme, Institute of Food Research, Colney, Norwich NR4 7UA, UK;Department of Food Engineering, Faculty of Engineering, Bayburt University, Bayburt, Turkey

关键词: Cell surface;    Adhesion;    Biofilm;    Exopolysaccharides;    Lactobacillus johnsonii;   

Others  :  1137660
DOI  :  10.1186/s12866-015-0347-2

 received in 2014-10-08, accepted in 2015-01-13,  发布年份 2015

【 摘 要 】

Background

The bacterial cell surface is a crucial factor in cell-cell and cell-host interactions. Lactobacillus johnsonii FI9785 produces an exopolysaccharide (EPS) layer whose quantity and composition is altered in mutants that harbour genetic changes in their eps gene clusters. We have assessed the effect of changes in EPS production on cell surface characteristics that may affect the ability of L. johnsonii to colonise the poultry host and exclude pathogens.

Results

Analysis of physicochemical cell surface characteristics reflected by Zeta potential and adhesion to hexadecane showed that an increase in EPS gave a less negative, more hydrophilic surface and reduced autoaggregation. Autoaggregation was significantly higher in mutants that have reduced EPS, indicating that EPS can mask surface structures responsible for cell-cell interactions. EPS also affected biofilm formation, but here the quantity of EPS produced was not the only determinant. A reduction in EPS production increased bacterial adhesion to chicken gut explants, but made the bacteria less able to survive some stresses.

Conclusions

This study showed that manipulation of EPS production in L. johnsonii FI9785 can affect properties which may improve its performance as a competitive exclusion agent, but that positive changes in adhesion may be compromised by a reduction in the ability to survive stress.

【 授权许可】

   
2015 Dertli et al.; licensee BioMed Central.

【 图 表 】

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