Drought resistant of bacteria producing exopolysaccharide and IAA in rhizosphere of soybean plant (Glycine max) in Wonogiri Regency Central Java Indonesia

4th International Conference on Sustainable Agriculture and Environment

农业科学;生态环境科学

Susilowati, A.^1 ; Puspita, A.A.^1 ; Yunus, A.^1

Bioscience Study Program, Graduate Program, Universitas Sebelas Maret, Surakarta, Indonesia^1

关键词: Bacillus licheniformis;    Bacillus megaterium;    Exopolysaccharides;    Isolation of bacteria;    Osmotic potential;    Resistant bacteria;    Sequence similarity;    Stress condition;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/142/1/012058/pdf
DOI  :  10.1088/1755-1315/142/1/012058

来源: IOP

【 摘 要 】

Drought is one of the main problem which limitating the agriculture productivity in most arid region such as in district Eromoko, Wuryantro and SelogiriWonogiri Central Java Indonesia. Bacteria are able to survive under stress condition by producte exopolysaccharide. This study aims to determine the presence of exopolysaccharide-producing drought-resistant bacteria on rhizosphere of soybean (Glycine max) and to determine the species of bacteria based on 16S rRNA gene. Isolation of bacteria carried out by the spread plate method. The decreased of osmotic potential for screening drought tolerant bacteria according to the previous equation [12]. Selection of exopolysaccharide-producing bacteria on solid media ATCC 14 followed by staining the capsule. 16S rRNA gene amplification performed by PCR using primers of 63f and 1387r. The identificationof the bacteria is determined by comparing the results of DNA sequence similarity with bacteria databank in NCBI database. The results showed 11 isolates were exopolysaccharide-producing drought tolerant bacteria. The identity of the bacteria which found are Bacillus sp, Bacillus licheniformis, Bacillus megaterium and Bacillus pumilus.