Identification of Ureolytic Bacteria for Concrete Formation and Antifungal Activity from the Soil with Long-Term Application of Urea Fertilizer

2018 4th International Conference on Environmental Science and Material Application

生态环境科学;材料科学

Pang, Haiyue^1 ; Wang, Gueyhorng^1^2 ; Yang, Hueian^3 ; Wu, Hongtan^1^2 ; Wang, Xinhui^3 ; Chen, Yupei^1^2^3

Application Technique Engineering Center of Natural Cosmeceuticals College of Fujian Province, Xiamen Medical College, Xiamen,Fujian

361023, China^1

Department of Medical Technology, Xiamen Medical College, Xiamen,Fujian

361023, China^2

Department of Biological Science and Technology, China University of Science and Technology, Taipei

115, Taiwan^3

关键词: 16S rRNA gene sequence;    Acetohydroxamic acids;    Anti-fungal activity;    Bacillus megaterium;    Degrading activities;    Genetic relationships;    Next-generation sequencing;    Random amplified polymorphic dnas;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/252/5/052131/pdf
DOI  :  10.1088/1755-1315/252/5/052131

来源: IOP

【 摘 要 】

Ureolytic bacteria in 12 isolates, namely TCU1-TCU12, obtained from the soil with long-term application of urea fertilizer were screened and characterized. Urea degrading activity of all isolates was ranging from 2.4 to 22.9 mM. The ureolysis profiles of the isolates corresponded to fluctuations in pH value. Urease inhibitors, including acetohydroxamic acid and phenyl phosphorodiamidate, were applied to prove urea degrading activity. Moreover, the antifungal activity was verified by the TCU4 and TCU5 strains. The 16S rRNA gene sequence analysis revealed that among the 12 isolates, eight were Bacillus aryabhattai, three were Bacillus megaterium, and one was Bacillus subtilis. The random amplified polymorphic DNA was used to detect the genetic relationship among the 12 isolates. In addition, next-generation sequencing indicated that Proteobacteria was the most abundant phylum at 47.1% in the soil with long-term application of urea fertilizer. Bacillus species occupied 0.12%. Consequently, these ureolytic bacteria had the potential to be applied in the fields of concrete structure and medicine.