New Medium for Isolation of Bacteria From Cement Kiln Dust with a Potential to Apply in Bio-Concrete

4th International Conference on Civil and Environmental Engineering for Sustainability

地球科学;生态环境科学;经济学

Alshalif, A.F.^1 ; Irwan, J.M.^1 ; Othman, N.^2 ; Al-Gheethi, A.^2

Jamilus Research Centre for Sustainable Construction (JRC), Faculty of Civil and Environmental Engineering, Universiti Tun Hussein Onn Malaysia, Parit Raja, Johor

86400, Malaysia^1

Micro-Pollutant Research Centre (MPRC), Faculty of Civil and Environmental Engineering, University Tun Hussein Onn Malaysia, Parit Raja, Johor

86400, Malaysia^2

关键词: Aerated concrete;    Bacterial isolates;    Bacterial strains;    Cement kiln dusts;    Chemical component;    Isolation of bacteria;    Morphological characteristic;    X ray fluorescence;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/140/1/012155/pdf
DOI  :  10.1088/1755-1315/140/1/012155

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

The present study aimed to introduce a new isolation medium named kiln dust medium (KDM) for recovering of bacteria from cement kiln dust with high pH (>pH 11) without the need for nutrients additives. The cement kiln dust samples were collected from five different areas of Cement Industries of Malaysia Berhad (CIMA). The bacterial isolates were recovered on KDM by direct plating technique. The chemical components for all collected samples were identified using X-ray fluorescence (XRF). The primary identification for the bacterial isolates indicated that these bacteria belongs to Bacillus spp. Based on the morphological characteristics. The growth curve of the bacterial strains was monitored using the optical density (OD) with 650 nm wavelength, which in role confirmed that all isolated bacteria had the ability to grow successfully in the proposed medium. The ability of the bacterial strains to grow at high pH reflects their potential in the bio-concrete applications (aerated and non-aerated concrete). These findings indicated that the cement kiln dust samples from Cement Industries represent the most appropriate source for bacteria used in the bioconcrete.