Antidepressant-like effects of a water-soluble extract from the culture medium of Ganoderma lucidum mycelia in rats

BMC Complementary and Alternative Medicine

Mari Okazaki²  Yasuhide Hibino¹  Hiroshi Iizuka³  Fumiko Suzuki³  Shinya Kamiuchi¹  Naohiro Iwata¹  Yuta Shimizu²  Hirokazu Matsuzaki² 

[1] Laboratory of Immunobiochemistry, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan;Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama 350-0295, Japan;Noda Shokukin-kogyo Co. Ltd.; 295 Nanakodai, Noda, Chiba 278-0051, Japan

关键词: 5-HT_2A receptors;    Memory;    Locomotion;    Ganoderma lucidum mycelia;    Depression;    Anxiety;   

Others  :  1220416
DOI  :  10.1186/1472-6882-13-370

 received in 2012-10-15, accepted in 2013-12-19,  发布年份 2013

【 摘 要 】

Background

Ganoderma lucidum is a popular medicinal mushroom used for promoting health and longevity in Asian countries. Previously, we reported that a water-soluble extract from a culture medium of Ganoderma lucidum mycelia (MAK) exerts antioxidative and cerebroprotective effects against ischemia–reperfusion injury in vivo. Here, we evaluated the antidepressant and anxiolytic activities of MAK in rats.

Methods

MAK (0.3 or 1 g/kg, p.o.) was administered in the experimental animals 60 min before the forced swimming, open-field, elevated plus-maze, contextual fear-conditioning, and head twitch tests. Additionally, the mechanisms involved in the antidepressant-like action of MAK were investigated by the serotonin precursor 5-hydroxy-L-tryptophan (5-HTP)- or 5-HT_2A agonist (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI)-induced head twitch responses.

Results

Treatment with MAK (1 g/kg) exhibited antidepressant-like effects in the forced swimming test, attenuated freezing behavior in the contextual fear-conditioning test, and decreased the number of head twitches induced by DOI, but not with 5-HTP. No significant response was observed in locomotion or anxiety-like behavior, when the animals were evaluated in the open-field or elevated plus-maze test, respectively.

Conclusions

These data suggest that MAK has antidepressant-like potential, which is most likely due to the antagonism of 5-HT_2A receptors, and possesses anxiolytic-like effects toward memory-dependent and/or stress-induced anxiety in rats.

【 授权许可】

   
2013 Matsuzaki et al.; licensee BioMed Central Ltd.

【 图 表 】

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