Antidepressant-like effects of a water-soluble extract from the culture medium of Ganoderma lucidum mycelia in rats

BMC Complementary and Alternative Medicine

Research Article

Yasuhide Hibino¹  Naohiro Iwata¹  Shinya Kamiuchi¹  Mari Okazaki²  Yuta Shimizu²  Hirokazu Matsuzaki²  Fumiko Suzuki³  Hiroshi Iizuka³ 

[1] Laboratory of Immunobiochemistry, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, 50-0295, Sakado, Saitama, Japan;Laboratory of Pharmacology, Faculty of Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, 350-0295, Sakado, Saitama, Japan;Noda Shokukin-kogyo Co. Ltd., 295 Nanakodai, 278-0051, Noda, Chiba, Japan;

关键词: Anxiety;    Depression;    Ganoderma lucidum;    Locomotion;    Memory;    5-HT receptors;   

DOI  :  10.1186/1472-6882-13-370

 received in 2012-10-15, accepted in 2013-12-19,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundGanoderma lucidum is a popular medicinal mushroom used for promoting health and longevity in Asian countries. Previously, we reported that a water-soluble extract from a culture medium of Ganoderma lucidum mycelia (MAK) exerts antioxidative and cerebroprotective effects against ischemia–reperfusion injury in vivo. Here, we evaluated the antidepressant and anxiolytic activities of MAK in rats.MethodsMAK (0.3 or 1 g/kg, p.o.) was administered in the experimental animals 60 min before the forced swimming, open-field, elevated plus-maze, contextual fear-conditioning, and head twitch tests. Additionally, the mechanisms involved in the antidepressant-like action of MAK were investigated by the serotonin precursor 5-hydroxy-L-tryptophan (5-HTP)- or 5-HT2A agonist (±)-1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane hydrochloride (DOI)-induced head twitch responses.ResultsTreatment with MAK (1 g/kg) exhibited antidepressant-like effects in the forced swimming test, attenuated freezing behavior in the contextual fear-conditioning test, and decreased the number of head twitches induced by DOI, but not with 5-HTP. No significant response was observed in locomotion or anxiety-like behavior, when the animals were evaluated in the open-field or elevated plus-maze test, respectively.ConclusionsThese data suggest that MAK has antidepressant-like potential, which is most likely due to the antagonism of 5-HT2A receptors, and possesses anxiolytic-like effects toward memory-dependent and/or stress-induced anxiety in rats.

【 授权许可】

Unknown   
© Matsuzaki et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.