Behavioral Changes in Combination Therapy of Ethanol and Modafinil on rats Focal Cerebral Ischemia

Basic and Clinical Neuroscience

ARTICLE

Abbasi, Yusef¹  Mousavizadeh, Kazem²  Shabani, Ronak²  Katebi, Majid⁵  Mehdizadeh, Mehdi² 

[1] Department of Anatomy, Faculty of Medicine, Arak University of Medical Sciences;Cellular and Molecular Research Center, Faculty of Medicine, Iran University of Medical Sciences;Department of Molecular Medicine, Faculty of Advanced Technologies in Medicine, Iran University of Medical Sciences;University of Medical Sciences, Tehran;Department of Anatomy, School of Medicine, Hormozgan University of Medical Sciences;Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences

关键词: Middle cerebral artery;    Ethanol;    Neuroprotection;    Locomotion;    Anxiety;   

DOI  :  10.32598/bcn.11.3.269

来源: Iran University of Medical Sciences

【 摘 要 】

Introduction: Ethanol is considered as an effective agent in reducing brain stroke injury. In this study, we assessed the effects of modafinil along with ethanol as a combination therapy on behavioral function in Wistar rats. Methods: The right Middle Cerebral Artery Occlusion (MCAO) was performed and the rats were divided into nine groups (n=8 per group). The animal groups in this study were as follows: 1. MCAO control group (ischemia without treatment); 2. Vehicle group; 3. Modafinil group that was randomly subdivided into three groups receiving different doses of modafinil (10, 30, and 100 mg/kg) for 7 days before MCAO; 4. Ethanol group receiving 1.5 g/kg ethanol at the time of reperfusion; 5. Modafinil + ethanol group that was further subdivided into three groups receiving modafinil at different doses (10, 30, and 100 mg/kg) for 7 days before MCAO and ethanol at the time of reperfusion. The motor behavior was measured using the Garcia test 24, 48, and 72 h after the ischemia, and the elevated body swing test was performed 48 and 72 h after the ischemia. The anxiety and locomotor activity were analyzed by open field test 48 and 72 h post-ischemia. Results: The results showed that the neurological deficit score, locomotor activity, and unexpected thigmotaxis (anxiety) in the ethanol, modafinil (in a dose-dependent manner), and ethanol+modafinil treatment groups were significantly higher than the MCAO control group. Conclusion: It seems that the combination therapy of modafinil (100 mg/kg) and ethanol (1.5 g/kg) significantly enhanced neuroprotection via an improvement in locomotor activity and neurological functions.

【 授权许可】

Unknown