BMC Evolutionary Biology | |
The evolution of antibiotic susceptibility and resistance during the formation of Escherichia coli biofilms in the absence of antibiotics | |
Luke J Harmon4  Larry J Forney4  Paul Joyce3  José M Ponciano1  Jabus G Tyerman2  | |
[1] Department of Biology, University of Florida, Gainesville, FL, USA;Current address: Genomatica, Inc., 10520 Wateridge Circle, San Diego, CA, 92121, USA;Departments of Statistics, University of Idaho, Moscow, ID, 83844, USA;Initiative for Bioinformatics and Evolutionary Studies (IBEST), University of Idaho, Moscow, ID, 83844, USA | |
关键词: Diversity; Mutations; Bacterial biofilms; Antibiotic resistance; Evolution; | |
Others : 1130095 DOI : 10.1186/1471-2148-13-22 |
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received in 2012-06-18, accepted in 2013-01-11, 发布年份 2013 | |
【 摘 要 】
Background
Explanations for bacterial biofilm persistence during antibiotic treatment typically depend on non-genetic mechanisms, and rarely consider the contribution of evolutionary processes.
Results
Using Escherichia coli biofilms, we demonstrate that heritable variation for broad-spectrum antibiotic resistance can arise and accumulate rapidly during biofilm development, even in the absence of antibiotic selection.
Conclusions
Our results demonstrate the rapid de novo evolution of heritable variation in antibiotic sensitivity and resistance during E. coli biofilm development. We suggest that evolutionary processes, whether genetic drift or natural selection, should be considered as a factor to explain the elevated tolerance to antibiotics typically observed in bacterial biofilms. This could be an under-appreciated mechanism that accounts why biofilm populations are, in general, highly resistant to antibiotic treatment.
【 授权许可】
2013 Tyerman et al.; licensee BioMed Central Ltd.
【 预 览 】
Files | Size | Format | View |
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20150226162947579.pdf | 395KB | download | |
Figure 3. | 65KB | Image | download |
Figure 2. | 58KB | Image | download |
Figure 1. | 64KB | Image | download |
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