Genetic characterization of Mycobacterium tuberculosis in the West Bank, Palestinian Territories

BMC Research Notes

Gila Kahila Bar-Gal⁴  Nalin Rastogi³  Mark Spigelman¹  Charles L Greenblatt²  Ziad Abdeen⁵  Kifaya Azmi⁵  Abedelmajeed Nasereddin⁵  Suheir Ereqat⁴ 

[1] Royal Free & University College Medical School, Centre for Clinical Microbiology, Royal Free Hospital, Rowland Hill Street, London, UK;Department of Microbiology and Molecular Genetics, The Hebrew University-Hadassah Medical School, Jerusalem, Israel;WHO Supranational TB Reference Laboratory, Unité de la Tuberculose et des Mycobactéries, Institut Pasteur de Guadeloupe, Abymes, Guadeloupe, France;Koret School of Veterinary Medicine, The Robert H. Smith faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot, 76100, Israel;Al-Quds Nutrition and Health Research Institute, Faculty of Medicine, Al-Quds University, P. O. Box: 201760, Abu-Deis, Palestine

关键词: Genotyping;    MIRU;    Spoligotyping;    Mycobacterium;    Tuberculosis;   

Others  :  1166364
DOI  :  10.1186/1756-0500-5-270

 received in 2012-03-05, accepted in 2012-05-30,  发布年份 2012

【 摘 要 】

Background

The World Health Organization (WHO) declared human tuberculosis (TB) a global health emergency and launched the “Global Plan to Stop Tuberculosis” which aims to save a million lives by 2015. Global control of TB is increasingly dependent on rapid and accurate genetic typing of species of the Mycobacterium tuberculosis (MTB) complex including M. tuberculosis. The aim of this study was to identify and genetically characterize the MTB isolates circulating in the West Bank, Palestinian Territories. Genotyping of the MTB isolates from patients with pulmonary TB was carried out using two molecular genetic techniques, spoligotyping and mycobacterial interspersed repetitive units-variable number of tandem repeat (MIRU-VNTR) supported by analysis of the MTB specific deletion 1 (TbD1).

Findings

A total of 17 MTB patterns were obtained from the 31 clinical isolates analyzed by spoligotyping; corresponding to 2 orphans and 15 shared-types (SITs). Fourteen SITs matched a preexisting shared-type in the SITVIT2 database, whereas a single shared-type SIT3348 was newly created. The most common spoligotyping profile was SIT53 (T1 variant), identified in 35.5 % of the TB cases studied. Genetic characterization of 22 clinical isolates via the 15 loci MIRU-VNTR typing distinguished 19 patterns. The 15-loci MIT144 and MIT145 were newly created within this study. Both methods determined the present of M. bovis strains among the isolates.

Conclusions

Significant diversity among the MTB isolates circulating in the West Bank was identified with SIT53-T1 genotype being the most frequent strain. Our results are used as reference database of the strains circulating in our region and may facilitate the implementation of an efficient TB control program.

【 授权许可】

   
2012 Ereqat et al.; Licensee BioMed Central Ltd.

【 图 表 】

Figure 1 .

Figure 2 .

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