Analysis of Antioxidant Enzyme Activity Involved in Ascorbate-glutathione Cycle in Green-flesh Kiwifruits

International Conference on Manufacturing Technology, Materials and Chemical Engineering

机械制造;材料科学;化学工业

Xia, Hui^1,2 ; Gao, Fan^1,2 ; Ni, Zhiyou^1,2 ; Liang, Dong^1,2

College of Horticulture, Sichuan Agricultural University, Chengdu

611130, China^1

Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu

611130, China^2

关键词: Antioxidant enzyme activity;    Ascorbate peroxidase;    Ascorbate-glutathione cycle;    Dehydroascorbate reductase;    Kiwifruits;    Ultraviolet spectrophotometry;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/392/5/052016/pdf
DOI  :  10.1088/1757-899X/392/5/052016

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

To compare the activities of enzymes related to ascorbate-glutathione cycle (AsA-GSH cycle) in different varieties, the fruits of five green-fleshed kiwifruit varieties were used as materials and activities of ascorbate peroxidase (APX), monodehydroascorbic acid (MDHA), dehydroascorbate reductase (DHAR) were measured by ultraviolet spectrophotometry. Results showed that 'Cuiyu' had higher MDHAR activities. The higher enzyme activity is responsible for the higher accumulation of AsA in kiwifruit. All of these results indicated that there were significant differences in the activities of enzymes in 5 kiwifruit genotypes.