A novel dehydroascorbate reductase from spinach chloroplasts homologous to plant trypsin inhibitor

FEBS Letters

Trümper, Susanne¹  Häberlein, Ingo¹  Follmann, Hartmut¹ 

[1] Biochemie, Fachbereich Biologie-Chemie der Universität Kassel, D-34109 Kassel, Germany

关键词: Chloroplast enzyme;    Dehydroascorbate reductase;    Glutaredoxin;    Spinach (Spinacia oleracea);    Thioredoxin;    Trypsin inhibitor;    DHA;    dehydroascorbate;    GSH;    GSSG;    reduced and oxidized glutathione;    MDA;    monodehydroascorbate;   

DOI  :  10.1016/0014-5793(94)00947-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dehydroascorbate reductase has been isolated from spinach chloroplasts and purified to apparent homogeneity. The N-terminal amino acid sequence of the enzyme is homologous to the Kunitz-type trypsin inhibitors from plant sources. It is shown that spinach DHA reductase and soybean trypsin inhibitor are both capable of reducing dehydroascorbate when in the reduced (thiol) form but acquire trypsin-inhibiting activity in the oxidized (disulfide) state. Reduced chloroplast thioredoxins also reduce dehydroascorbate.

【 授权许可】

Unknown