Antioxidant metabolic system and comparative proteomics analysis in winter turnip rape (Brassica rapa L.) under cold stress

Oil Crop Science

Wancang Sun¹  Li Ma²  Yaping Xie³  Jinhai Yuan⁴  Tianxia Guan⁵  Xiucun Zeng⁵  Xiaoqin Wang⁵  Guotai Ma⁵  Yaozhao Xu⁵ 

[1] Corresponding author.;College of Agronomy, Gansu Agricultural University, Lanzhou, 730070, China;Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou, 730070, China;Linze County Seed Administration, Zhangye, 734000, China;School of Life Science and Engineering/ College of Agriculture and Ecological Engineering / Key Laboratory of Hexi Corridor Resources Utilization of Gansu, Hexi University, Zhangye, 734000, China;

关键词: Brassica rapa;    Accumulation of H2O2 and O2•-;    Antioxidant enzyme activity;    Cold stress;   

DOI  :  

来源: DOAJ

【 摘 要 】

Winter turnip rape (Brassica rapa L.) is widely cultivated in winter in Northwest China, however, its cold-tolerant mechanism remains insufficiently understood. In this study, winter turnip rape cultivar Longyou 7, a cold-tolerant variety, was used as material, whose accumulation of H2O2 and O2•-, antioxidant enzyme activity as well as differences in protein expression based on two-dimensional gel electrophoresis (2-DE) technique under −4°C stress were analyzed. Results showed that, production of H2O2 and O2•- were increased in Longyou 7 leaves, simultaneously, SOD and POD activities were also obviously rosed up, but the activities of CAT and APX were gradually reduced with the temperature. Thirty-six differential protein spots were successfully identified between control and treatments group by using mass spectrometry analysis. Among them, 4 differential protein spots were induced under cold stress, and 2 were inhibited at −4°C. Functional analysis found that these identified proteins mainly participated in photosynthesis, carbohydrate metabolism, defense, protein synthesis, enzyme activity, redox and membrane metabolism, respectively. Additionally, 13 proteins' function were still unknown. In conclusion, strong antioxidant capacity and cell defense ability might play important roles in Longyou 7 response to cold stress.

【 授权许可】

Unknown