Effects of Streptomycin Wastewater on the Activity of GSH-PX in Zebrafish before and after Aerobic Biological Treatment

2018 2nd International Conference on Environmental and Energy Engineering

生态环境科学;能源学

Liu, Yi Nan^1 ; Shen, Hong Yan^1

College of Environmental Science and Engineering, Pharmaceutical Molecular Chemistry Laboratory of Hebei Province, Hebei University of Science and Technology, Shijiazhuang, China^1

关键词: Aerobic biological treatment;    Anaerobic methods;    Antioxidant enzyme activity;    Antioxidant systems;    Exposure groups;    Oxidative damage;    Secondary treatment;    Wastewater treatment plants;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/146/1/012057/pdf
DOI  :  10.1088/1755-1315/146/1/012057

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Most wastewater treatment plants now use aerobic biological treatment to dispose wastewater, but the toxicity of influent and effluent is unclear. In this study, subacute toxicity test was used to set four exposure groups and one control group to measure GSH-PX activity when zebrafish was exposed for 3,6,9,12,15 days at the indicated volume ratio, which is 10%, 30%, 50%, 70% respectively for influent and 10%, 20%, 30%, 40% for effluent. For influent, because of the response to the oxidative stress, the GSH-PX activity was activated in all other exposure groups except for the 70% volume group. The GSH-PX activity in 70% volume was significantly lower than the control group indicates that because of the sustaining increase of volume ratio, antioxidant system in zebrafish was destroyed and antioxidant enzyme activity was reduced. But for effluent, GSH-PX activity in each group was less than that in influent but still slightly induced, which means that after aerobic biological treatment, the sewage can reduce oxidative damage to zebrafish, but still have induction effect. For both influent and effluent, maximum of GSH-PX activity was both at the 30% exposure volume (18.7523U/mgprot for influent, 10.8023 U/mgprot for influent) which can provide the data basis for future research. All in all, GSH-PX activity in zebrafish muscle tissue can response to the oxidative stress and the changes can be used as an indicator of oxidative damage. And it is necessary to use the anaerobic method for the secondary treatment.