Changes of antioxidant enzyme activities in chloroplast of '717' poplar under NaCl treatment

2nd International Symposium on Resource Exploration and Environmental Science

生态环境科学

Teng, Qingyun^1

School of Nanjing Forestry University, China^1

关键词: 717 hybrid yang;    Antioxidant enzyme;    Antioxidant enzyme activity;    Antioxidant systems;    Low concentrations;    Peroxidase (POD);    Reduced glutathione;    Salt stresss;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/170/5/052004/pdf
DOI  :  10.1088/1755-1315/170/5/052004

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

In order to study the salt tolerance of '717' hybrid poplar under salt treatment, we studied the changes of antioxidant enzyme activities in chloroplasts treated with 0, 25,75mmol/L Knack. The results showed that: (1) Knack treatment can increase the content of hydrogen peroxide (H2O2) and malondialdehyde (MDA) in chloroplast. The H2O2 increased the highest at 1d, and reached 39.5%. The content of MDA in chloroplast increased by 38.3% at the low concentration of day 5, and increased to 74.4% at day 10 after high concentration of Knack treatment. (2) The activity of acerbate peroxidase (APX) in chloroplast increased at low concentration and decreased at high concentration. The activity of peroxidase (POD) in chloroplast was not significantly changed at low concentration, but at high concentration, the POD activity decreased by up to 35% at day 1. The activity of glutathione reductive (GR) in chloroplast was increased by 15.9% under low Knack treatment. Increased by 53.6% at 1 d on high concentration of Knack. (3) Knack treatment, the activities of ascorbic acid (As A) and (reduced glutathione) GSH in chloroplast were increased. At low concentration, the activity of ASA in chloroplast increased by 43.8% at 10 d, and the content of GSH was an increase of 72.2% at 10 d. In the high concentration of Knack treatment, the activity increased significantly. In conclusion, the low concentration of Knack (25mmol / L) could promote the activity of antioxidant enzymes in chloroplast of 717 and maintain the growth, while the antioxidant enzyme activity was inhibited when the Knack was treated with high concentration (75mmol / L). Hydrogen peroxide and MDA content increased, the cells were subjected to Knack stress.