Ferrous Ion and Medium Composition Effects on Acidogenic Phase in Biobutanol Production from Molasses

International Conference on Energy Sciences 2016

能源学;物理学

Restiawaty, E.^1,2 ; Grinanda, D.^2

Study Program of Bioenergy and Chemurgy Engineering, Research Group of Chemical Engineering Process Design and Development, Faculty of Industrial Technology, Institut Teknologi Bandung, Jln. Let. Jen. Purn. Dr. (HC) Mashudi No. 1, Sumedang, West Java

45363, Indonesia^1

Biosciences and Biotechnology Research Centre, Institut Teknologi Bandung, Jl. Ganesha 10, Bandung, West Java

40132, Indonesia^2

关键词: Acetic acid concentration;    Acidogenic;    Clostridium acetobutylicum;    Fermentation medium;    Fermentation process;    Ferrous ion;    Product selectivities;    Raw material costs;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/877/1/012072/pdf
DOI  :  10.1088/1742-6596/877/1/012072

来源: IOP

【 摘 要 】

Clostridium acetobutylicum B530 has ability to convert sugar into biobutanol through two phases, i.e. acidogenic and solventogenic. This fermentation process is often hampered by high raw material cost and low product yield. In order to suppress the production cost, the molasses, a byproduct of sugar cane process production, was used as carbon source in this research. Molasses has nitrogen content in a small amount, thus could be negating the beef extract component, which is expected not to affect the growth of C. acetobutylicum B530 and also can reduce the production cost. In addition, a certain amount of Fe2+(ferrous ion), a precursor in the formation of the enzyme ferredoxin, was added to the fermentation medium to contribute in the synthesis of acetyl-CoA, so that the formation of acidogenic products such as butyric acid and acetic acid is affected. This study aimed to investigate the effect of ferrous ion and the medium composition in acidogenic phase. The addition of 20 ppm FeSO4.7H2O in the fermentation medium without beef extract can increase the concentration of butyric acid by 20% at a temperature of 35°C, while acetic acid concentration decreased by 6%. According to those results, it is expected that the product selectivity of butanol will increase in solventogenic phase. In addition, the removal of beef extract in the fermentation medium does not affect the kinetics of growth of C. acetobutylicum B530.