Response surface method optimization of ectoine fermentation medium with moderate halophilic bacteria Halomonas sp. H02

3rd International Conference on Agricultural and Biological Sciences

农业科学;生物科学

Li, T.T.^1 ; Qu, A.^1 ; Yuan, X.N.^1 ; Tan, F.X.^1 ; Li, X.W.^1 ; Wang, T.^1 ; Zhang, L.H.^1

Collaborative Innovation Center for Vessel Pollution Monitoring, Control at Dalian Maritime University, Dalian

116026, China^1

关键词: Fermentation medium;    Halophilic bacteria;    High osmotic stress;    NaCl concentration;    Optimum combination;    Response surface method;    Response surface methodology;    Small organic molecules;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/77/1/012019/pdf
DOI  :  10.1088/1755-1315/77/1/012019

来源: IOP

【 摘 要 】

Moderate halophilic bacteria are of halophilic bacteria whose suitable growth of NaCl is 5-10%. When the moderate halophilic bacteria response to high osmotic stress, the intracellular will synthesize small organic molecule compatible solutes. Ectoine, which is the major synthetic osmotic compatible solutes for moderate halophilic bacteria, can help microbial enzymes, nucleic acids and the whole cell resist to hypertonic, high temperature, freezing and other inverse environment. In order to increase the Ectoine production of Moderate halophilic bacteria Halomonas sp. H02, the Ectoine fermentation medium component was optimized by Plackett-Burman (PB) and Response Surface Methodology (RSM) based on the principle of non-complete equilibrium The results of PB experiments showed that the three main influencing factors of Moderate halophilic bacteria Halomonas sp. H02 synthesis Ectoine culture medium were C5H8NNaO4concentration, NaCl concentration and initial pH. According to the center point of the steepest climbing experiment, the central combination design experiment was used to show that the model is consistent with the actual situation. The optimum combination of three influencing factors were C5H8NNaO441 g/L, NaCl 87.2 g/L and initial pH 5.9, and the predicted amount of Ectoine was 1835.8 mg/L, increased by 41.6%.