Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose

Proteome Science

Mark G Lefsrud²  Nathan C Verberkmoes¹  Robert L Hettich¹  Manesh Shah¹  Vijaya GS Raghavan²  Kumaran Sivagnanam² 

[1] Oak Ridge National Laboratory, Chemical and Life Sciences Divisions, Oak Ridge, TN, USA;Department of Bioresource Engineering, Macdonald Campus, McGill University, Quebec, Canada

关键词: mass spectrometry;    shotgun proteomics;    Clostridium acetobutylicum;    ABE fermentation;    Butanol;   

Others  :  820177
DOI  :  10.1186/1477-5956-9-66

 received in 2011-06-06, accepted in 2011-10-18,  发布年份 2011

【 摘 要 】

Background

Butanol is a second generation biofuel produced by Clostridium acetobutylicum through acetone-butanol-ethanol (ABE) fermentation process. Shotgun proteomics provides a direct approach to study the whole proteome of an organism in depth. This paper focuses on shotgun proteomic profiling of C. acetobutylicum from ABE fermentation using glucose and xylose to understand the functional mechanisms of C. acetobutylicum proteins involved in butanol production.

Results

We identified 894 different proteins in C. acetobutylicum from ABE fermentation process by two dimensional - liquid chromatography - tandem mass spectrometry (2D-LC-MS/MS) method. This includes 717 proteins from glucose and 826 proteins from the xylose substrate. A total of 649 proteins were found to be common and 22 significantly differentially expressed proteins were identified between glucose and xylose substrates.

Conclusion

Our results demonstrate that flagellar proteins are highly up-regulated with glucose compared to xylose substrate during ABE fermentation. Chemotactic activity was also found to be lost with the xylose substrate due to the absence of CheW and CheV proteins. This is the first report on the shotgun proteomic analysis of C. acetobutylicum ATCC 824 in ABE fermentation between glucose and xylose substrate from a single time data point and the number of proteins identified here is more than any other study performed on this organism up to this report.

【 授权许可】

   
2011 Sivagnanam et al; licensee BioMed Central Ltd.

【 图 表 】

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