Proteome Science | |
Comparative shotgun proteomic analysis of Clostridium acetobutylicum from butanol fermentation using glucose and xylose | |
Research | |
Vijaya GS Raghavan1  Kumaran Sivagnanam1  Mark G Lefsrud1  Nathan C Verberkmoes2  Manesh Shah2  Robert L Hettich2  | |
[1] Department of Bioresource Engineering, McGill University, Macdonald Campus, Quebec, Canada;Oak Ridge National Laboratory, Chemical and Life Sciences Divisions, Oak Ridge, TN, USA; | |
关键词: Butanol; ABE fermentation; Clostridium acetobutylicum; shotgun proteomics; mass spectrometry; | |
DOI : 10.1186/1477-5956-9-66 | |
received in 2011-06-06, accepted in 2011-10-18, 发布年份 2011 | |
来源: Springer | |
【 摘 要 】
BackgroundButanol is a second generation biofuel produced by Clostridium acetobutylicum through acetone-butanol-ethanol (ABE) fermentation process. Shotgun proteomics provides a direct approach to study the whole proteome of an organism in depth. This paper focuses on shotgun proteomic profiling of C. acetobutylicum from ABE fermentation using glucose and xylose to understand the functional mechanisms of C. acetobutylicum proteins involved in butanol production.ResultsWe identified 894 different proteins in C. acetobutylicum from ABE fermentation process by two dimensional - liquid chromatography - tandem mass spectrometry (2D-LC-MS/MS) method. This includes 717 proteins from glucose and 826 proteins from the xylose substrate. A total of 649 proteins were found to be common and 22 significantly differentially expressed proteins were identified between glucose and xylose substrates.ConclusionOur results demonstrate that flagellar proteins are highly up-regulated with glucose compared to xylose substrate during ABE fermentation. Chemotactic activity was also found to be lost with the xylose substrate due to the absence of CheW and CheV proteins. This is the first report on the shotgun proteomic analysis of C. acetobutylicum ATCC 824 in ABE fermentation between glucose and xylose substrate from a single time data point and the number of proteins identified here is more than any other study performed on this organism up to this report.
【 授权许可】
Unknown
© Sivagnanam et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
【 预 览 】
Files | Size | Format | View |
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